Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody

Product Name

Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody

View all MAP2K1 Antibodies

SKU/Catalog Number

MP00292

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody (Catalog # MP00292). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00292)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 17C9.E1.H2.D7.H7.D5

Isotype

IgG1 lambda

Immunogen

Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

MP00292 is reactive to MAP2K1 in Human, Mouse, Rat

Applications

MP00292 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

43.439kDa

Background of MAP2K1

MEK1 pS222/MEK2 pS226 antibody detects MEK1 and MEK2. Mitogen-activated protein kinase kinase 1, (also known as MKK or MEK1), and Mitogen-activated protein kinase kinase 2, (also known as MEK2 or MKK2), are integral components of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 and 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:40,000
WB: User optimized

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MAP2K1/MAP2K2 using anti-MAP2K1/MAP2K2 antibody (MP00292).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1/MAP2K2 antigen affinity purified polyclonal antibody (Catalog # MP00292) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for MAP2K1/MAP2K2.

Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)

Alternative Names

dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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